Protein Purification
Sigma Aldrich Fine Chemicals Biosciences Protease and Phosphatase Inhibitor Cocktail | 1ML
Protease and Phosphatase Inhibitor Cocktail | 1ML
Invent Biotechnologies Inc Minute™ Cytoplasmic & Nuclear Extraction Kits for Cells - Spin Column-Based < 15 min (50 Preps)
Minute™ Cytoplasmic and Nuclear Extraction Kit for Cells is composed of optimized cytoplasmic extraction buffer, nuclear protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly separate native cytosol and nuclear proteins from mammalian cells and protoplasts of plant, bacteria, yeast and fungus. Separation of cytoplasmic and nuclear proteins can be accomplished in less than 15 minutes using the protein extraction filter cartridges.
STEMCELL Technologies EasySep™ Human Gamma/Delta T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Gamma/Delta T Cell Isolation Kit is designed to isolate gamma/delta T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-gamma/delta T cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube. To obtain a custom kit optimized for culture-expanded PBMCs, contact STEMCELL Technologies’ Technical Support at techsupport@stemcell.com.
Vector Laboratories Glycoprotein Eluting Solution for GlcNAc or Chitin-Binding Lectins 100 ml
This Glycoprotein Eluting Solution can be used to elute glycoproteins from agarose-bound N-acetylglucosamine- or chitin-binding lectins. The neutral pH of this solution maximizes the yield of eluted glycoproteins and preserves the activity of the agarose-bound lectins for repeated use. All components of this ready-to-use solution can be subsequently removed by dialysis. This Glycoprotein Eluting Solution offers convenience and superior recovery over standard sugar solutions.
Andwin Scientific MG132 1MG
MG-132 is a chemical that performs as a cell-permeable, potent, reversible proteasome inhibitor. This product is a white solid chemical that is in powder form. It has a Health Hazard NFPA Rating of 0, a Fire Hazard NFPA Rating of 1, and a Reactivity NFPA Rating of 0. This is packaged and sold in a 1 milligram glass bottle.
STEMCELL Technologies "The Big Easy" EasySep™ Magnet, For isolating: 1 Unit
"The Big Easy" EasySep™ Magnet is designed for cell separation procedures using EasySep™ reagents when processing a large number of cells, or when using RoboSep™. "The Big Easy" EasySep™ Magnet generates a high-gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with EasySep™ Magnetic Particles without the use of columns. "The Big Easy" EasySep™ Magnet is designed to hold a standard 14 mL (17 x 95 mm) polystyrene tube.
Norgen Biotek Corp Cytoplasmic & Nuclear RNA Purification Kit, 50 Prep
This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol. Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations. Ten samples can be processed in approximately 45 minutes. This kit is also available in a 100 prep size.
STEMCELL Technologies EasySep™ Direct Human Eosinophil Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Eosinophil Isolation Kit isolates functional, highly purified eosinophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation, or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.
Bioassay Systems Phosphatase Inhibitor. Sodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems vanadate reagent is activated for maximum inhibition. Uses: to preserve protein phosphorylation state in cells and lysates.
Sodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems vanadate reagent is activated for maximum inhibition. Example of uses: preserve protein phosphorylation state in cells and lysates, and use as a standard phosphatase inhibitor in phosphatase assays. Key Features: Ready-to-use phosphatase inhibitor. Fully activated for maximum inhibition. Kit size: 1 mL 100 mM. Sodium Orthovanadate (CAS Number 13721-39-6, Na3VO4) is a general inhibitor for protein phosphotyrosyl phosphatases (PTPs). Inhibition of vanadate is competitive and can be reversed by addition of EDTA or by dilution. Vanadate is commonly used to preserve the protein tyrosyl phosphorylation state in cells and lysates. BioAssay Systems Vanadate reagent is activated for maximum inhibition. Suggested Working Concentration: 1-10 mM
Sigma Aldrich Fine Chemicals Biosciences SIGMAFAST(TM) Protease Inhibitor Cocktail Tablets, EDTA-Free for use in purification of Histidine-tagged proteins | MFCD00677817 | 2TAB
SIGMAFAST(TM) Protease Inhibitor Cocktail Tablets, EDTA-Free for use in purification of Histidine-tagged proteins | MFCD00677817 | 2TAB